Reduced sputum expression of interferon-stimulated genes in severe COPD.

ABSTRACT:

Background

Exacerbations of COPD are frequent and commonly triggered by respiratory tract infections. The purpose of our study was to investigate innate immunity in stable COPD patients.

Methods

Induced sputum was collected from 51 stable consecutive COPD patients recruited from the COPD Clinic of CHU Liege and 35 healthy subjects. Expression of interferons beta (IFN-β) and lambda1 (IL-29), IFN-stimulated genes (ISGs) MxA, OAS, and viperin were measured in total sputum cells by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The presence of Picornaviruses was assessed by RT-PCR, while potential pathogenic microorganisms (PPM) were identified by sputum bacteriology.

Results

Expression of IL-29 was found in 16 of 51 COPD patients (31%) and in nin

SUBMITTER: Hilzendeger C 

PROVIDER: S-EPMC4934534 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature