Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets.

ABSTRACT: CD8(+) T cells develop increased sensitivity following Ag experience, and differences in sensitivity exist between T cell memory subsets. How differential TCR signaling between memory subsets contributes to sensitivity differences is unclear. We show in mouse effector memory T cells (TEM) that >50% of lymphocyte-specific protein tyrosine kinase (Lck) exists in a constitutively active conformation, compared with <20% in central memory T cells (TCM). Immediately proximal to Lck signaling, we observed enhanced Zap-70 phosphorylation in TEM following TCR ligation compared with TCM Furthermore, we observed superior cytotoxic effector function in TEM compared with TCM, and we provide evidence that this results from a lower probability of TCM reaching threshold signaling owing to the decreased ma

SUBMITTER: Moogk D 

PROVIDER: S-EPMC4935560 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature