A native promoter and inclusion of an intron is necessary for efficient expression of GFP or mRFP in Armillaria mellea.

ABSTRACT: Armillaria mellea is a significant pathogen that causes Armillaria root disease on numerous hosts in forests, gardens and agricultural environments worldwide. Using a yeast-adapted pCAMBIA0380 Agrobacterium vector, we have constructed a series of vectors for transformation of A. mellea, assembled using yeast-based recombination methods. These have been designed to allow easy exchange of promoters and inclusion of introns. The vectors were first tested by transformation into basidiomycete Clitopilus passeckerianus to ascertain vector functionality then used to transform A. mellea. We show that heterologous promoters from the basidiomycetes Agaricus bisporus and Phanerochaete chrysosporium that were used successfully to control the hygromycin resistance cassette were not able to support expr

SUBMITTER: Ford KL 

PROVIDER: S-EPMC4935854 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature