An algorithm for estimation and correction of anisotropic magnification distortion of cryo-EM images without need of pre-calibration.

ABSTRACT: Anisotropic magnification distortion of TEM images (mainly the elliptic distortion) has been recently found as a potential resolution-limiting factor in single particle 3-D reconstruction. Elliptic distortions of ∼1-3% have been reported for multiple microscopes under low magnification settings (e.g., 18,000×), which significantly limited the achievable resolution of single particle 3-D reconstruction, especially for large particles. Here we report a generic algorithm that formulates the distortion correction problem as a generalized 2-D alignment task and estimates the distortion parameters directly from the particle images. Unlike the present pre-calibration methods, our computational method is applicable to all datasets collected at a broad range of magnifications using any microscope w

SUBMITTER: Yu G 

PROVIDER: S-EPMC4935934 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature