Efficient Sulfide Assimilation in Methanosarcina acetivorans Is Mediated by the MA1715 Protein.

ABSTRACT:

Unlabelled

Conserved genes essential to sulfur assimilation and trafficking in aerobic organisms are missing in many methanogens, most of which inhabit highly sulfidic, anaerobic environmental niches. This suggests that methanogens possess distinct pathways for the synthesis of key metabolites and intermediates, including cysteine, homocysteine, and protein persulfide groups. Prior work identified a novel tRNA-dependent two-step pathway for cysteine biosynthesis and a new metabolic transformation by which sulfur is inserted into aspartate semialdehyde to produce homocysteine. Homocysteine biosynthesis requires two of the three proteins previously identified in our laboratory by a comprehensive bioinformatics approach. Here, we show that the third protein identified in silico, the A

SUBMITTER: Rauch BJ 

PROVIDER: S-EPMC4936107 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature