Classical V600E and other non-hotspot BRAF mutations in adult differentiated thyroid cancer.

ABSTRACT:

Background

BRAF is the most frequently mutated gene in differentiated thyroid cancer (DTC). Previous studies on DTC have well documented high rates of the BRAF (V600E) mutation in patients of mixed ages. Previous studies either included a mix of pediatric and adult patients or pediatric patients only. However, the prevalence of hotspot and non-hotspot BRAF mutations and its significance in pure adult DTCs is not yet well determined. In this study we determine the frequency of this classical BRAF mutation and other rare BRAF mutations in pure adult DTCs.

Methods

A total of 204 adult DTC samples (Age >18 years) were analyzed for mutations in exon 15 of the BRAF gene by performing polymerase chain reaction (PCR) amplification of tumor genomic DNAs and direct sequencing of ampli

SUBMITTER: Murugan AK 

PROVIDER: S-EPMC4936197 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature