A Reliable Protocol for In situ microRNAs Detection in Feeding Sites Induced by Root-Knot Nematodes.

ABSTRACT: Galls induced by Meloidogyne spp. in plant roots are a complex organ formed by heterogeneous tissues; within them there are 5-8 giant cells (GCs) that root-knot nematodes use for their own nurturing. Subtle regulatory mechanisms likely mediate the massive gene repression described at early infection stages in galls, particularly in giant cells. Some of these mechanisms are mediated by microRNAs (miRNAs); hence we describe a reliable protocol to detect miRNAs abundance within the gall tissues induced by Meloidogyne spp. Some methods are available to determine the abundance of specific miRNAs in different plant parts; however, galls are complex organs formed by different tissues. Therefore, detection of miRNAs at the cellular level is particularly important to understand specific regulatory

SUBMITTER: Diaz-Manzano FE 

PROVIDER: S-EPMC4936241 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature