Tumor evolution and progression in multifocal and paired non-invasive/invasive urothelial carcinoma.

ABSTRACT: Although multifocal tumors and non-invasive/invasive components are commonly encountered in surgical pathology, their genetic relationship is often poorly characterized. We used next-generation sequencing (NGS) to characterize somatic alterations in a patient with five spatially distinct, high-grade papillary urothelial carcinomas (UCs), with one tumor harboring an underlying invasive component. NGS of 409 cancer-related genes was performed on DNA isolated from formalin-fixed paraffin-embedded (FFPE) blocks representing each papillary tumor (n = 5), the invasive component of one tumor, and matched normal tissue. We identified nine unique non-synonymous somatic mutations across the six UC samples, including five present in each carcinoma sample, consistent with clonal origin and limited int

SUBMITTER: Warrick JI 

PROVIDER: S-EPMC4936404 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature