Circulating Tumor Cell Phenotype Predicts Recurrence and Survival in Pancreatic Adenocarcinoma.

ABSTRACT:

Objective

We assessed circulating tumor cells (CTCs) with epithelial and mesenchymal phenotypes as a potential prognostic biomarker for patients with pancreatic adenocarcinoma (PDAC).

Background

PDAC is the fourth leading cause of cancer death in the United States. There is an urgent need to develop biomarkers that predict patient prognosis and allow for better treatment stratification.

Methods

Peripheral and portal blood samples were obtained from 50 patients with PDAC before surgical resection and filtered using the Isolation by Size of Epithelial Tumor cells method. CTCs were identified by immunofluorescence using commercially available antibodies to cytokeratin, vimentin, and CD45.

Results

Thirty-nine patients (78%) had epithelial CTCs that expressed cytoke

SUBMITTER: Poruk KE 

PROVIDER: S-EPMC4936958 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature