Transcriptional reprogramming of metabolic pathways in critically ill patients.

ABSTRACT:

Background

Critical illness causes a shift away from mitochondrial metabolism towards a greater dependence on glycolysis. This metabolic shift is thought to be associated with lactic acidosis, organ dysfunction and poor clinical outcomes. The current paradigm is that low oxygen supply causes regional hypoxia, which in turn drives such a metabolic shift. In this study, we evaluated whether the shift towards glycolysis can also occur in cells where oxygen supply is plentiful.

Methods

We used circulating blood cells from non-hypoxic critically ill patients (n = 47) as a model to study cellular metabolism in a normal oxygen milieu. We measured the transcriptomic profiles of canonical metabolic pathways in these cells and compared them to cells obtained from healthy controls (n =

SUBMITTER: Nalos M 

PROVIDER: S-EPMC4936987 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature