A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes.

ABSTRACT: Aberrant modifications of proteins occur during disease development and elicit disease-specific antibody responses. We have developed a protein array platform that enables the modification of many proteins in parallel and assesses their immunogenicity without the need to express, purify, and modify proteins individually. We used anticitrullinated protein antibodies (ACPAs) in rheumatoid arthritis (RA) as a model modification and profiled antibody responses to ∼190 citrullinated proteins in 20 RA patients. We observed unique antibody reactivity patterns in both clinical anticyclic citrullinated peptide assay positive (CCP+) and CCP- RA patients. At individual antigen levels, we detected antibodies against known citrullinated autoantigens and discovered and validated five novel antibodies ag

SUBMITTER: Karthikeyan K 

PROVIDER: S-EPMC4937507 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature