Control of the collective migration of enteric neural crest cells by the Complement anaphylatoxin C3a and N-cadherin.

ABSTRACT: We analyzed the cellular and molecular mechanisms governing the adhesive and migratory behavior of enteric neural crest cells (ENCCs) during their collective migration within the developing mouse gut. We aimed to decipher the role of the complement anaphylatoxin C3a during this process, because this well-known immune system attractant has been implicated in cephalic NCC co-attraction, a process controlling directional migration. We used the conditional Ht-PA-cre transgenic mouse model allowing a specific ablation of the N-cadherin gene and the expression of a fluorescent reporter in migratory ENCCs without affecting the central nervous system. We performed time-lapse videomicroscopy of ENCCs from control and N-cadherin mutant gut explants cultured on fibronectin (FN) and micropatterned FN-

SUBMITTER: Broders-Bondon F 

PROVIDER: S-EPMC4937886 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature