Residualization Rates of Near-Infrared Dyes for the Rational Design of Molecular Imaging Agents.

ABSTRACT:

Purpose

Near-infrared (NIR) fluorescence imaging is widely used for tracking antibodies and biomolecules in vivo. Clinical and preclinical applications include intraoperative imaging, tracking therapeutics, and fluorescent labeling as a surrogate for subsequent radiolabeling. Despite their extensive use, one of the fundamental properties of NIR dyes, the residualization rate within cells following internalization, has not been systematically studied. This rate is required for the rational design of probes and proper interpretation of in vivo results.

Procedures

In this brief report, we measure the cellular residualization rate of eight commonly used dyes encompassing three core structures (cyanine, boron-dipyrromethene (BODIPY), and oxazine/thiazine/carbopyronin).

Results

SUBMITTER: Cilliers C 

PROVIDER: S-EPMC4938006 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature