Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy.

ABSTRACT: Single Molecule Localization super-resolution Microscopy (SMLM) has become a powerful tool to study cellular architecture at the nanometer scale. In SMLM, single fluorophore labels are made to repeatedly switch on and off ("blink"), and their exact locations are determined by mathematically finding the centers of individual blinks. The image quality obtainable by SMLM critically depends on efficacy of blinking (brightness, fraction of molecules in the on-state) and on preparation longevity and labeling density. Recent work has identified several combinations of bright dyes and imaging buffers that work well together. Unfortunately, different dyes blink optimally in different imaging buffers, and acquisition of good quality 2- and 3-color images has therefore remained challenging. In this s

SUBMITTER: Nahidiazar L 

PROVIDER: S-EPMC4938622 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature