Fluorescent Beads Are a Versatile Tool for Staging Caenorhabditis elegans in Different Life Histories.

ABSTRACT: Precise staging of Caenorhabditis elegans is essential for developmental studies in different environmental conditions. In favorable conditions, larvae develop continuously through four larval stages separated by molting periods. Distinguishing molting from intermolt larvae has been achieved using transgenes with molting reporters, therefore requiring strain constructions, or careful observation of individuals for pharyngeal pumping or behavioral quiescence. In unfavorable conditions, larvae can enter the stress-resistant and developmentally arrested dauer larva stage. Identifying dauer larvae has been based on their ability to withstand detergent selection, precluding identification of recovering animals or of mutants with defects in dauer morphogenesis. Here, we describe a simple method

SUBMITTER: Nika L 

PROVIDER: S-EPMC4938646 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature