Identifying Genetic Signatures of Natural Selection Using Pooled Population Sequencing in Picea abies.

ABSTRACT: The joint inference of selection and past demography remain a costly and demanding task. We used next generation sequencing of two pools of 48 Norway spruce mother trees, one corresponding to the Fennoscandian domain, and the other to the Alpine domain, to assess nucleotide polymorphism at 88 nuclear genes. These genes are candidate genes for phenological traits, and most belong to the photoperiod pathway. Estimates of population genetic summary statistics from the pooled data are similar to previous estimates, suggesting that pooled sequencing is reliable. The nonsynonymous SNPs tended to have both lower frequency differences and lower FST values between the two domains than silent ones. These results suggest the presence of purifying selection. The divergence between the two domains base

SUBMITTER: Chen J 

PROVIDER: S-EPMC4938651 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature