Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus.

ABSTRACT:

Background

Bombyx mori bidensovirus (BmBDV), which belongs to the Bidnaviridae family established by the International Committee on Taxonomy of Viruses in 2011, was the first bidensovirus identified in insects. The structure of BmBDV is similar to that of parvoviruses, while its replication is similar to that of adenoviruses. Although BmBDV has the potential to be used as a tool in biological pest control and as an expression vector, virus rescue has been a bottleneck in the application of this virus.

Methods

In this study, we constructed a full-length genomic clone of BmBDV and showed that its terminal structure was restored. A recombinant BmBDV that expressed the green fluorescence protein (GFP) gene was constructed. Then, BmN cells, which are an ovarian cell line, were co

SUBMITTER: Zhang P 

PROVIDER: S-EPMC4938981 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature