Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine.

ABSTRACT: Lung engineering is a promising technology, relying on re-seeding of either human or xenographic decellularized matrices with patient-derived pulmonary cells. Little is known about the species-specificity of decellularization in various models of lung regeneration, or if species dependent cell-matrix interactions exist within these systems. Therefore decellularized scaffolds were produced from rat, pig, primate and human lungs, and assessed by measuring residual DNA, mechanical properties, and key matrix proteins (collagen, elastin, glycosaminoglycans). To study intrinsic matrix biologic cues, human endothelial cells were seeded onto acellular slices and analyzed for markers of cell health and inflammation. Despite similar levels of collagen after decellularization, human and primate lungs

SUBMITTER: Balestrini JL 

PROVIDER: S-EPMC4939101 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature