A divide-and-conquer approach to determine the Pareto frontier for optimization of protein engineering experiments.

ABSTRACT: In developing improved protein variants by site-directed mutagenesis or recombination, there are often competing objectives that must be considered in designing an experiment (selecting mutations or breakpoints): stability versus novelty, affinity versus specificity, activity versus immunogenicity, and so forth. Pareto optimal experimental designs make the best trade-offs between competing objectives. Such designs are not "dominated"; that is, no other design is better than a Pareto optimal design for one objective without being worse for another objective. Our goal is to produce all the Pareto optimal designs (the Pareto frontier), to characterize the trade-offs and suggest designs most worth considering, but to avoid explicitly considering the large number of dominated designs. To do so,

SUBMITTER: He L 

PROVIDER: S-EPMC4939273 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature