A Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand Interactions.

ABSTRACT: The key steps in cellular signaling and regulatory pathways rely on reversible noncovalent protein-ligand binding, yet the equilibrium parameters for such events remain challenging to characterize and quantify in solution. Here, we demonstrate a microfluidic platform for the detection of protein-ligand interactions with an assay time on the second timescale and without the requirement for immobilization or the presence of a highly viscous matrix. Using this approach, we obtain absolute values for the electrophoretic mobilities characterizing solvated proteins and demonstrate quantitative comparison of results obtained under different solution conditions. We apply this strategy to characterize the interaction between calmodulin and creatine kinase, which we identify as a novel calmodulin ta

SUBMITTER: Herling TW 

PROVIDER: S-EPMC4939750 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature