Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

ABSTRACT: Near infrared (NIR) photography and video was investigated as a method for observing and recording intrapuparial development in the tsetse fly Glossina palpalis gambiensis and other Muscomorpha (Cyclorrhapha) Diptera. We showed that NIR light passes through the puparium, permitting images of the true pupae and pharate adult to be captured. Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs. This study was carried out to advance our understanding of tsetse pupal development, specifically with the goal of improving a sorting technique which could separate male from female tsetse flies several days before emergence. Separation of the sexes at this stage is high

SUBMITTER: Moran ZR 

PROVIDER: S-EPMC4940448 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature