Analysis of Translation Elongation Dynamics in the Context of an Escherichia coli Cell.

ABSTRACT: Understanding the mechanisms behind translation and its rate-limiting steps is crucial for both the development of drug targets and improvement of heterologous protein production with many biotechnological applications, such as in pharmaceutical and biofuel industries. Despite many advances in the knowledge of the ribosome structure and function, there is still much discussion around the determinants of translation elongation with experiments and computational studies pointing in different directions. Here, we use a stochastic framework to simulate the process of translation in the context of an Escherichia coli cell by gathering the available biochemical data into a ribosome kinetics description. Our results from the study of translation in E. coli at different growth rates contradict the

SUBMITTER: Vieira JP 

PROVIDER: S-EPMC4940617 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature