CD248/endosialin critically regulates hepatic stellate cell proliferation during chronic liver injury via a PDGF-regulated mechanism.

ABSTRACT:

Introduction

CD248 (endosialin) is a stromal cell marker expressed on fibroblasts and pericytes. During liver injury, myofibroblasts are the main source of fibrotic matrix.

Objective

To determine the role of CD248 in the development of liver fibrosis in the rodent and human setting.

Design

CD248 expression was studied by immunostaining and quantitative PCR in both normal and diseased human and murine liver tissue and isolated hepatic stellate cells (HSCs). Hepatic fibrosis was induced in CD248(-/-) and wild-type controls with carbon tetrachloride (CCl4) treatment.

Results

Expression of CD248 was seen in normal liver of humans and mice but was significantly increased in liver injury using both immunostaining and gene expression assays. CD248 was co-expressed wit

SUBMITTER: Wilhelm A 

PROVIDER: S-EPMC4941145 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature