Multiplex amplicon sequencing for microbe identification in community-based culture collections.

ABSTRACT: Microbiome analysis using metagenomic sequencing has revealed a vast microbial diversity associated with plants. Identifying the molecular functions associated with microbiome-plant interaction is a significant challenge concerning the development of microbiome-derived technologies applied to agriculture. An alternative to accelerate the discovery of the microbiome benefits to plants is to construct microbial culture collections concomitant with accessing microbial community structure and abundance. However, traditional methods of isolation, cultivation, and identification of microbes are time-consuming and expensive. Here we describe a method for identification of microbes in culture collections constructed by picking colonies from primary platings that may contain single or multiple micr

SUBMITTER: Armanhi JS 

PROVIDER: S-EPMC4941570 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature