The receptor preference of influenza viruses.

ABSTRACT:

Objectives

The cell surface receptor used by an influenza virus to infect that cell is an N-acetyl neuraminic acid (NANA) residue terminally linked by an alpha2,3 or alpha2,6 bond to a carbohydrate moiety of a glycoprotein or glycolipid. Our aim was to determine a quick and technically simple method to determine cell receptor usage by whole influenza A virus particles.

Methods

We employed surface plasmon resonance to detect the binding of viruses to fetuin, a naturally occurring glycoprotein that has both alpha2,3- and alpha2,6-linked NANA, and free 3'-sialyllactose or 6'-sialyllactose to compete virus binding. All virus stocks were produced in embryonated chicken's eggs.

Results

The influenza viruses tested bound preferentially to NANAalpha2,3Gal or to NANAalpha2,6Ga

SUBMITTER: Meng B 

PROVIDER: S-EPMC4941662 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature