Molecular and phylogenetic analysis of matrix gene of avian influenza viruses isolated from wild birds and live bird markets in the USA.

ABSTRACT:

Background

Wild birds are the natural hosts for influenza A viruses (IAVs) and provide a niche for the maintenance of this virus.

Objectives

This study was undertaken to analyze nucleotide sequences of the matrix (M) gene of AIVs isolated from wild birds and live bird markets (LBMs) to index the changes occurring in this gene.

Methods

M-gene of 229 avian influenza virus (AIV) isolates obtained from wild birds and LBMs was amplified and sequenced. Full-length sequences (∼900 nt.) thus obtained were analyzed to identify changes that may be associated with resistance to adamantanes. Phylogenetic analysis of all sequences was performed using clustalw, and evolutionary distances were calculated by maximum composite likelihood method using mega (ver. 5.0) software.

Resul

SUBMITTER: Chander Y 

PROVIDER: S-EPMC4941746 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature