Resurfaced cell-penetrating nanobodies: A potentially general scaffold for intracellularly targeted protein discovery.

ABSTRACT: By virtue of their size, functional group diversity, and complex structure, proteins can often recognize and modulate disease-relevant macromolecules that present a challenge to small-molecule reagents. Additionally, high-throughput screening and evolution-based methods often make the discovery of new protein binders simpler than the analogous small-molecule discovery process. However, most proteins do not cross the lipid bilayer membrane of mammalian cells. This largely limits the scope of protein therapeutics and basic research tools to those targeting disease-relevant receptors on the cell surface or extracellular matrix. Previously, researchers have shown that cationic resurfacing of proteins can endow cell penetration. However, in our experience, many proteins are not amenable to such

SUBMITTER: Bruce VJ 

PROVIDER: S-EPMC4941773 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature