Oligonucleotide microchip for subtyping of influenza A virus.

ABSTRACT:

Background

Influenza A viruses are classified into subtypes depending on the antigenic properties of their two outer glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Sixteen subtypes of HA and nine of NA are known. Lately, the circulation of some subtypes (H7N7, H5N1) has been closely watched because of the epidemiological threat they present.

Objectives

This study assesses the potential of using gel-based microchip technology for fast and sensitive molecular subtyping of the influenza A virus.

Methods

The method employs a microchip of 3D gel-based elements containing immobilized probes. Segments of the HA and NA genes are amplified using multiplex RT-PCR and then hybridized with the microchip.

Results

The developed microchip was validated using a pane

SUBMITTER: Fesenko EE 

PROVIDER: S-EPMC4941880 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature