A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of synapses.

ABSTRACT: Intercellular protein-protein interactions (PPIs) enable communication between cells in diverse biological processes, including cell proliferation, immune responses, infection, and synaptic transmission, but they are challenging to visualize because existing techniques have insufficient sensitivity and/or specificity. Here we report a split horseradish peroxidase (sHRP) as a sensitive and specific tool for the detection of intercellular PPIs. The two sHRP fragments, engineered through screening of 17 cut sites in HRP followed by directed evolution, reconstitute into an active form when driven together by an intercellular PPI, producing bright fluorescence or contrast for electron microscopy. Fusing the sHRP fragments to the proteins neurexin (NRX) and neuroligin (NLG), which bind each othe

SUBMITTER: Martell JD 

PROVIDER: S-EPMC4942342 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature