Difference in Membrane Repair Capacity Between Cancer Cell Lines and a Normal Cell Line.

ABSTRACT: Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique, providing a sensitive index of repair capacity. The normal primary cell line of all tested cell lines exhibited the slowest rate of dye entry after laser disruption and lowest level of dye uptake. Significant

SUBMITTER: Frandsen SK 

PROVIDER: S-EPMC4942495 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature