Identification of candidate genes for myeloma-induced osteocyte death based on microarray data.

ABSTRACT:

Background

The study was aimed to investigate the molecular mechanisms of osteocyte death in multiple myeloma (MM) patients.

Methods

GSE27372 was downloaded from Gene Expression Omnibus, including three HOB-01 (osteocyte cell line) control samples and three HOB-01 samples co-cultured with JJN3 (human MM cell line). After the differentially expressed genes (DEGs) were identified by Student's t test method, enrichment analyses were performed for them using DAVID software. Using TRANSFAC, TSGene, and tumor-associated gene (TAG) databases, functional annotation was conducted for the DEGs. Additionally, protein-protein interaction (PPI) network and sub-network analyses were performed using STRING database and Cytoscape software.

Results

Total 393 DEGs were identified, incl

SUBMITTER: Tian H 

PROVIDER: S-EPMC4942932 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature