Development of a two-step protocol for culture expansion of human annulus fibrosus cells with TGF-β1 and FGF-2.

ABSTRACT:

Background

Different biologic approaches to treat disc regeneration, including growth factors (GFs) application, are currently under investigation. Human annulus fibrosus (hAF) repair or regeneration is one of the key elements for maintenance and restoration of nucleus pulposus function. However, so far there is no effective treatment for this purpose. The aim of the present study was to investigate the response of hAF cells to different combinations of GFs, and develop a protocol for efficient culture expansion.

Methods

hAF cells were harvested from degenerated disc tissues during surgical intervertebral disc removal, and hAF cells were expanded in a monolayer. The experiments were categorized based on different protocols with transforming growth factor (TGF-β1) and fibrobl

SUBMITTER: Chou PH 

PROVIDER: S-EPMC4942939 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature