Identification of human viral protein-derived ligands recognized by individual MHCI-restricted T-cell receptors.

ABSTRACT: Evidence indicates that autoimmunity can be triggered by virus-specific CD8(+) T cells that crossreact with self-derived peptide epitopes presented on the cell surface by major histocompatibility complex class I (MHCI) molecules. Identification of the associated viral pathogens is challenging because individual T-cell receptors can potentially recognize up to a million different peptides. Here, we generate peptide length-matched combinatorial peptide library (CPL) scan data for a panel of virus-specific CD8(+) T-cell clones spanning different restriction elements and a range of epitope lengths. CPL scan data drove a protein database search limited to viruses that infect humans. Peptide sequences were ranked in order of likelihood of recognition. For all anti-viral CD8(+) T-cell clones exam

SUBMITTER: Szomolay B 

PROVIDER: S-EPMC4943067 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature