The Role of Transcription Factors at Antisense-Expressing Gene Pairs in Yeast.

ABSTRACT: Genes encoded close to one another on the chromosome are often coexpressed, by a mechanism and regulatory logic that remain poorly understood. We surveyed the yeast genome for tandem gene pairs oriented tail-to-head at which expression antisense to the upstream gene was conserved across species. The intergenic region at most such tandem pairs is a bidirectional promoter, shared by the downstream gene mRNA and the upstream antisense transcript. Genomic analyses of these intergenic loci revealed distinctive patterns of transcription factor regulation. Mutation of a given transcription factor verified its role as a regulator in trans of tandem gene pair loci, including the proximally initiating upstream antisense transcript and downstream mRNA and the distally initiating upstream mRNA. To inv

SUBMITTER: Mostovoy Y 

PROVIDER: S-EPMC4943177 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature