Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing.

ABSTRACT: Demands for faster and more accurate methods to analyze microbial communities from natural and clinical samples have been increasing in the medical and healthcare industry. Recent advances in next-generation sequencing technologies have facilitated the elucidation of the microbial community composition with higher accuracy and greater throughput than was previously achievable; however, the short sequencing reads often limit the microbial composition analysis at the species level due to the high similarity of 16S rRNA amplicon sequences. To overcome this limitation, we used the nanopore sequencing platform to sequence full-length 16S rRNA amplicon libraries prepared from the mouse gut microbiota. A comparison of the nanopore and short-read sequencing data showed that there were no significa

SUBMITTER: Shin J 

PROVIDER: S-EPMC4944186 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature