Visualization and Sequencing of Membrane Remodeling Leading to Influenza Virus Fusion.

ABSTRACT:

Unlabelled

Protein-mediated membrane fusion is an essential step in many fundamental biological events, including enveloped virus infection. The nature of protein and membrane intermediates and the sequence of membrane remodeling during these essential processes remain poorly understood. Here we used cryo-electron tomography (cryo-ET) to image the interplay between influenza virus and vesicles with a range of lipid compositions. By following the population kinetics of membrane fusion intermediates imaged by cryo-ET, we found that membrane remodeling commenced with the hemagglutinin fusion protein spikes grappling onto the target membrane, followed by localized target membrane dimpling as local clusters of hemagglutinin started to undergo conformational refolding. The local dimples

SUBMITTER: Gui L 

PROVIDER: S-EPMC4944294 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature