Ontology highlight
ABSTRACT:
SUBMITTER: Tirosh I
PROVIDER: S-EPMC4944528 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20160401 6282
To explore the distinct genotypic and phenotypic states of melanoma tumors, we applied single-cell RNA sequencing (RNA-seq) to 4645 single cells isolated from 19 patients, profiling malignant, immune, stromal, and endothelial cells. Malignant cells within the same tumor displayed transcriptional heterogeneity associated with the cell cycle, spatial context, and a drug-resistance program. In particular, all tumors harbored malignant cells from two distinct transcriptional cell states, such that t ...[more]