Technical Advances of the Recombinant Antibody Microarray Technology Platform for Clinical Immunoproteomics.

ABSTRACT: In the quest for deciphering disease-associated biomarkers, high-performing tools for multiplexed protein expression profiling of crude clinical samples will be crucial. Affinity proteomics, mainly represented by antibody-based microarrays, have during recent years been established as a proteomic tool providing unique opportunities for parallelized protein expression profiling. But despite the progress, several main technical features and assay procedures remains to be (fully) resolved. Among these issues, the handling of protein microarray data, i.e. the biostatistics parts, is one of the key features to solve. In this study, we have therefore further optimized, validated, and standardized our in-house designed recombinant antibody microarray technology platform. To this end, we addressed

SUBMITTER: Delfani P 

PROVIDER: S-EPMC4944972 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature