Iterative sorting of apical and basolateral cargo in Madin-Darby canine kidney cells.

ABSTRACT: For several decades, the trans-Golgi network (TGN) was considered the most distal stop and hence the ultimate protein-sorting station for distinct apical and basolateral transport carriers that reach their respective surface domains in the direct trafficking pathway. However, recent reports of apical and basolateral cargoes traversing post-Golgi compartments accessible to endocytic ligands before their arrival at the cell surface and the post-TGN breakup of large pleomorphic membrane fragments that exit the Golgi region toward the surface raised the possibility that compartments distal to the TGN mediate or contribute to biosynthetic sorting. Here we describe the development of a novel assay that quantitatively distinguishes different cargo pairs by their degree of colocalization at the TG

SUBMITTER: Treyer A 

PROVIDER: S-EPMC4945143 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature