Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction.

ABSTRACT: Many aspects of chromatin biology are influenced by the nuclear compartment in which a locus resides, from transcriptional regulation to DNA repair. Further, the dynamic and variable localization of a particular locus across cell populations and over time makes analysis of a large number of cells critical. As a consequence, robust and automatable methods to measure the position of individual loci within the nuclear volume in populations of cells are necessary to support quantitative analysis of nuclear position. Here, we describe a three-dimensional membrane reconstruction approach that uses fluorescently tagged nuclear envelope or endoplasmic reticulum membrane marker proteins to precisely map the nuclear volume. This approach is robust to a variety of nuclear shapes, providing greater bi

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC4945324 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature