TRPA1 activation leads to neurogenic vasodilatation: involvement of reactive oxygen nitrogen species in addition to CGRP and NO.

ABSTRACT:

Background and purpose

Transient receptor potential ankyrin-1 (TRPA1) activation is known to mediate neurogenic vasodilatation. We investigated the mechanisms involved in TRPA1-mediated peripheral vasodilatation in vivo using the TRPA1 agonist cinnamaldehyde.

Experimental approach

Changes in vascular ear blood flow were measured in anaesthetized mice using laser Doppler flowmetry.

Key results

Topical application of cinnamaldehyde to the mouse ear caused a significant increase in blood flow in the skin of anaesthetized wild-type (WT) mice but not in TRPA1 knockout (KO) mice. Cinnamaldehyde-induced vasodilatation was inhibited by the pharmacological blockade of the potent microvascular vasodilator neuropeptide CGRP and neuronal NOS-derived NO pathways. Cinnamaldehyde-me

SUBMITTER: Aubdool AA 

PROVIDER: S-EPMC4945766 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature