Hydrogen peroxide induced cell death: One or two modes of action?

ABSTRACT: Imlay and Linn show that exposure of logarithmically growing Escherichia coli to hydrogen peroxide (H2O2) leads to two kinetically distinguishable modes of cell killing. Mode one killing is pronounced near 1 mM concentration of H2O2 and is caused by DNA damage, whereas mode-two killing requires higher concentration ([Formula: see text]). The second mode seems to be essentially due to damage to all macromolecules. This phenomenon has also been observed in Fenton in vitro systems with DNA nicking caused by hydroxyl radical ([Formula: see text]). To our knowledge, there is currently no mathematical model for predicting mode one killing in vitro or in vivo after H2O2 exposure. We propose a simple model, using Escherichia coli as a model organism and a set of ordinary differential equations. Us

SUBMITTER: Uhl L 

PROVIDER: S-EPMC4945851 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature