Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training.

ABSTRACT: We report a toolbox for exploring the modular tuning of genetic circuits, which has been specifically optimized for widespread deployment in STEM environments through a combination of bacterial strain engineering and distributable hardware development. The transfer functions of 16 genetic switches, programmed to express a GFP reporter under the regulation of the (acyl-homoserine lactone) AHL-sensitive luxR transcriptional activator, can be parametrically tuned by adjusting high/low degrees of transcriptional, translational, and post-translational processing. Strains were optimized to facilitate daily large-scale preparation and reliable performance at room temperature in order to eliminate the need for temperature controlled apparatuses, which are both cost-limiting and space-constraining.

SUBMITTER: Magaraci MS 

PROVIDER: S-EPMC4947003 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature