Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

ABSTRACT:

Objective

The objective of this study was to determine the contribution of lymphatic tissue to heterotopic ossification (HO).

Background

HO is the pathologic development of ectopic bone within soft tissues often following severe trauma. Characterization of the tissue niche supporting HO is critical to identifying therapies directed against this condition. Lymphangiogenesis is upregulated during incidents of trauma, thereby coincident with the niche supportive of HO. We hypothesized that lymphatic tissues play a critical role in HO formation.

Methods

Mice underwent hindlimb Achilles' tendon transection and dorsal burn injury (burn/tenotomy) to induce HO. The popliteal and inguinal lymph nodes were excised ipsilateral to the tenotomy site. Flow cytometry and immunostain

SUBMITTER: Loder S 

PROVIDER: S-EPMC4947025 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature