Impact of storage conditions on metabolite profiles of sputum samples from persons with cystic fibrosis.

ABSTRACT:

Background

Although recent studies have begun to elucidate how airway microbial community structure relates to lung disease in cystic fibrosis (CF), microbial community activity and the host's response to changes in this activity are poorly understood. Metabolomic profiling provides a means to investigate microbial activity and human cell activity within diseased airways. However, variables in sample storage and shipping likely affect downstream analyses and standards for sample handling are lacking.

Methods

We assessed the impact of sample storage conditions on liquid chromatography mass spectrometry analysis of CF sputum samples.

Results

Significant changes in global metabolomic profiles occurred in samples stored at room temperature or at 4°C for longer than one da

SUBMITTER: Zhao J 

PROVIDER: S-EPMC4947390 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature