Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells.

ABSTRACT: Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infectio

SUBMITTER: Frensing T 

PROVIDER: S-EPMC4947482 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature