Identification of differentially expressed genes in salivary adenoid cystic carcinoma cells associated with metastasis.

ABSTRACT:

Introduction

Salivary adenoid cystic carcinoma (SACC) is a frequent type of salivary gland cancer which is characterized by slow growth but high incidence of distant metastasis. We aimed to identify therapeutic targets which are associated with metastasis of SACC.

Material and methods

Total RNA was isolated from a low metastatic SACC cell line (ACC-2) and a highly metastatic SACC cell line (ACC-M), which was screened from ACC-2 by combination of in vivo selection and cloning in vitro. Then the total RNA was subjected to microarray analysis. Differentially expressed genes (DEGs) were screened from ACC-M compared with ACC-2, followed by Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Function annotation for DEGs also was p

SUBMITTER: Chen W 

PROVIDER: S-EPMC4947631 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature