A novel approach for next-generation sequencing of circulating tumor cells.

ABSTRACT:

Background

Next-generation sequencing (NGS) of surgically resected solid tumor samples has become integral to personalized medicine approaches for cancer treatment and monitoring. Liquid biopsies, or the enrichment and characterization of circulating tumor cells (CTCs) from blood, can provide noninvasive detection of evolving tumor mutations to improve cancer patient care. However, the application of solid tumor NGS approaches to circulating tumor samples has been hampered by the low-input DNA available from rare CTCs. Moreover, whole genome amplification (WGA) approaches used to generate sufficient input DNA are often incompatible with blood collection tube preservatives used to facilitate clinical sample batching.

Methods

To address this, we have developed a novel approach

SUBMITTER: Yee SS 

PROVIDER: S-EPMC4947859 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature