Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic.

ABSTRACT:

Purpose

Tissue-engineered colon (TEC) might potentially replace absent or injured large intestine, but the enteric nervous system (ENS), a key component, has not been investigated. In various enteric neuropathic diseases in which the TEC is derived from aganglionic donor colon, the resulting construct might also be aganglionic, limiting tissue engineering applications in conditions such as Hirschsprung disease (HD). We hypothesized that TEC might contain a diverse population of enteric neuronal subtypes, and that aganglionic TEC can be populated by neurons and glia when supplemented with ENS progenitor cells in the form of neurospheres.

Materials and methods

Human and murine organoid units (OU) and multicellular clusters containing epithelium and mesenchyme were isolated fro

SUBMITTER: Wieck MM 

PROVIDER: S-EPMC4948202 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature