Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells.

ABSTRACT:

Aim

To investigate in vitro the therapeutic effect and mechanisms of silybin in a cellular model of hepatic steatosis.

Methods

Rat hepatoma FaO cells were loaded with lipids by exposure to 0.75 mmol/L oleate/palmitate for 3 h to mimic liver steatosis. Then, the steatotic cells were incubated for 24 h with different concentrations (25 to 100 μmol/L) of silybin as phytosome complex with vitamin E. The effects of silybin on lipid accumulation and metabolism, and on indices of oxidative stress were evaluated by absorption and fluorescence microscopy, quantitative real-time PCR, Western blot, spectrophotometric and fluorimetric assays.

Results

Lipid-loading resulted in intracellular triglyceride (TG) accumulation inside lipid droplets, whose number and size increased. TG a

SUBMITTER: Vecchione G 

PROVIDER: S-EPMC4948277 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature